Separation of Some Drugs by HPLC using New Cross linked Co-polymer of (Trietyl amine glyceryl maleate)

Abstract

A rigid, easy grinded and highly stable cross linked poly (triethyl amine glyceryl malate) was synthesized by condensation polymerization of a mixture of triethanol amine and glycerol with maleic anhydride. The synthesized polymer was used as a stationary phase for HPLC to analyze some drugs like furosemide, atenolol, and amiloride. The study investigate the effect of phosphate buffer concentration and pH on the analysis of the drugs. The practical pH for the drug separation was found to be 8, whereas, the eluent was 20% phosphate buffer and 80% methanol giving good analysis results using isocratic elution. The retention times for furosemide, atenolol, and amiloride were 4.64min, 7.05min, and 9.16min, respectively. The separation was improved by using elution gradient (0-20)% phosphate buffer in methanol, flow rate1ml/min and wavelength 233nm. High recoveries were obtained for the drugs, in the range from 95-98%, by using the new column. The obtained results were compared with commercial column ODS-C18 and the method of analysis was extended for determination of the drugs in pharmaceutical tablets as well as in serum samples.