Effect of Royal jelly in genotoxic and cytotoxic effect of Fumagillin in white mice

Abstract

This study was conducted in Laboratories of college of science, Tikrit University, and Faculty of science, Dohok University, Iraq between 2014 and 2016. The aim of study was to evaluate the potential of royal jelly to ameliorates genotoxic and cytotoxic effects of Fumagillin in white mice Mus musculus. Fumagillin which produced by Aspergillus fumigates is a natural antibiotic used in veterinary against Microsporidia parasites in bees and fish. It also used in human medicine for AIDS patients and other immune dysfunction. In this current study we evaluate genotoxicity of Fumagillin by micronucleus test in bone marrow cells, chromosome aberration in primary spermatocytes and cytotoxicity by mitotic index in bone marrow cells of white mice, and study the activity of royal jelly in removing genotoxic and cytotoxic effects of Fumagillin. The fumagillin was given as repeated oral gavages for 7 successive days after preparing the doses 10, 15, and 20 mg/kg.b.wt. in 50% of sugar syrup. The positive control group was given single I.p. dose of 20 mg/kg.b.wt. of methotrexate. 100 mg of royal jelly alone and with each dose of Fumagillin was given to evaluate the effect of royal jelly in toxic effect of Fumagillin. The results showed that the two dosages 15 and 20 mg/kg.b.wt of Fumagillin induced significant increase in the frequency of micronuclei, the means was ($24.60 \pm 2.37$, $53.00 \pm 4.59$) respectively compared with negative control ($8.20 \pm 1.39$), and significant increase in the means of total chromosome aberration in bone marrow cells of the mice in the these groups ($9.00 \pm 0.92, 17.20 \pm 1.24$) respectively compared with ($6.00 \pm 0.83$) for negative control. In primary spermatocytes, the dose 20mg/kg.b.wt. of fumagillin induced significant increase in the mean of chromosome aberration ($14.00 \pm 2.07$) compared with ($3.90 \pm 0.50$) for negative control. The three dosages 10, 15, and 20