Determine the antibacterial activity of lemon (Citrus limon) extract against some bacteria isolated from different sources

Abstract

The antibacterial activity of lemon extract (Citrus limon) was investigated. The aim of the study was to determine antibacterial activity of lemon juice against some pathogenic bacteria isolated from different clinical samples. Agar well diffusion assay was used to detect antibacterial activity of different concentration of lemon juice (25, 50, 75, and 100)%, and some antibiotics against Staphylococcus aureus, Klebseilla pneumoniae, Proteus mirabilis, Escherichia coli, and Pseudomonas aeruginosa. The results revealed, all isolates were inhibited by 100% lemon juice with Diameter of Inhibition Zone (DIZ) of (13-23) mm. The inhibitory activity of lemon juice was found to be higher than the effects exerted by some used antibiotics except MEM, and NOR which inhibited Pseudomonas aeruginosa with DIZ of (19-30)mm, and(18-19)mm respectively. Also, RA, and NV which were inhibited Staphylococcus aureus with DIZ (25-28)mm, and (27-31)mm respectively. Inaddition to CIP that produced DIZ of (19, 23, and 28) mm against Escherichia coli, Klebseilla pneumoniae, and Proteus mirabilis respectively. it can be concluded that lemon juice extract has substantial antibacterial activity, and possible to be used for health promotion, accessory to the antibiotic therapies, since it is inexpensive, natural, and had less side effects. further studies regarding antibacterial activity of lemon juice against pathogenic bacteria in vivo is recommended.