Spectrophotometric determination of Ampicillin and Lansoprazole in pure forms and in their pharmaceutical Formulations

Abstract

Accurate, rapid and economical methods were used to estimate the two drugs: ampicillin and lansoprazole, using the reagent "4-chloro-7-nitrobenzofurazane" (NBD-Cl) in the alkaline medium, these methods depend on the formation of colored products between those drugs and the chromogenic reagent (NBD-Cl). at (pH 10.8) it has the highest absorption at 468 nm for Ampicillin (AMP), as well as a yellow colored product and at (pH 11.3) and has the highest absorption at 470 nm for Lansoprazole (LPZ). Beer's law was applied in a range of concentrations (3.6-32.4), (3.6-24) μg/ml, with an molar absorptivity (6.908×10³L/mol. cm) (1.086×10⁴L/mol. cm), and the correlation factor (0.9961, 0.9984) respectively, and the limits of detection were (1.511μg/ml), (0.588μg/ml), respectively. The proposed methods have been successfully applied to estimate these drugs in their pure form and in their pharmaceutical preparations such as injections and capsules in ampicillin and lansoprazole respectively.