The Therapeutic Evaluation of Extracted Chitosan from Local Fungus Truffle Against Hyperlipidemia Induced In Male Rabbits

Abstract

The present study aimed to identify the therapeutic evaluation of chitosan extracted from the fungus truffle and pure chitosan on glucose and lipid profile in the blood of male rabbits with hyperlipidemia induced experimentally by cholesterol. The tests included estimation of glucose levels, total cholesterol, triglycerides, High-density lipoproteins, low-density lipoproteins, and very low-density lipoproteins. Hyperlipidemia was induced in the male rabbits used in the study, which was administered orally with cholesterol 150mg / kg body weight. Male rabbits were divided into eight groups: control, Cholesterol, Pure chitosan, Brown truffle chitosan, White truffle chitosan, Cholesterol and pure chitosan, Cholesterol and brown truffle chitosan and Cholesterol and white truffle chitosan. The results of the study showed, The hyperlipidemia induced experimentally resulted a significant increase (P<0.05) in the concentrations of glucose, TC, TG, LDL, and VLDL, while no significant difference in HDL compared with control group. The treatment of male rabbits with pure chitosan resulted in no significant differences in glucose, HDL. While there was a significant decrease (P<0.05) in TC, TG, LDL, and VLDL compared with control group. The treatment of animals with cholesterol and pure chitosan led to a significant increase (P<0.05) in HDL, while a significant decrease (P<0.05) in glucose, TC, TG, LDL, and VLDL, compared with cholesterol group. When the rabbits were treated with brown truffles chitosan, there was no significant difference in glucose, HDL, while there was a significant decrease (P<0.05) in TC, TG, LDL, and VLDL compared with the control group. The treatment with cholesterol and white truffle chitosan, showed increase (P<0.05) significant in HDL, while there was a significant decrease (P<0.05) in TC, TG, LDL, and VLDL, compared with the cholesterol group. When rabbits were the treated with cholesterol and brown truffles chitosan, led to a significant increase (P<0.05) in HDL, significant decrease (P<0.05) in TC, TG, LDL, and VLDL, while there was no significant difference in glucose compared with the cholesterol group. The treatment with cholesterol and white truffle chitosan, showed increase (P<0.05) significant in HDL, while there was a significant decrease (P<0.05) in TC, TG, LDL, and VLDL, compared with the cholesterol group. It is evident from the current study that the extracted chitosan has an important role to reduce the high levels of fats and their complications.