Study of genotoxic and cytotoxic effects of tobacco and Urethane in lung and colorectal of white mice

Abstract

The genotoxic and cytotoxic effects of tobacco and urethane were studied and the results using the cytogenetic endpoints: micronucleus test (MN) in polychromatic erythrocytes (PCEs), chromosome aberration analysis in the bone-marrow of white mice Mus musculus. In this observation,, The impact of various doses of cigarette smoke and mouse lung urethane on the lung and the colorectal morphological changes, both in the lung and in the colorectum, is further examined. The cigar mice were then exposed to smoke once or twice a day in a locked environment five times a week. urethane after injection of Interpretation (5mg / kg). The effect of various doses of mouse lung and colorectal tobacco smoke and urethane-induced mouse lung also tests morphological modifications in both the lung and colorectal of the mice. The animals were divided into six groups at random, while the negative (control) group got distal water only. The results of the statistical analysis indicated that micronucleus immature blood cells in the bone marrow had a significant increase of P ≤ 0.05 for both urethane and tobacco treatment in the formation of micronuclei compared to negative control. The study findings also indicated that the induction of chromosomal aberration of immature red blood cells in the bone marrow significantly increased by P ≤ 0.05. Also the results showed number Tobacco treatment and urethane induced histological changes in both lung and colorectal tissue of nodules and hyperplasia amongst these various groups, as revealed by light microscope. In conclusion, current study revealed that tobacco and Urethane have high genotoxicity and Cytogenetically effects on somatic cells of white mice, also ever smokers have an increased risk of lung and colon cancer.