The effect of some antibiotics and antiseptics on several bacterial genera isolated from the animal house

Abstract

From different places of animal house (University of Baghdad/ biology department/ college of science), that include cages of (rats, mice and rabbits), walls, float, counters and sinks, the specimens were taken by using 25 sterile swabs. The kinds of isolated bacteria were identified according to the morphological features, biochemical tests and by VITEK2 system. fourteen strains were isolated belonged to Pseudomonas aeruginosa, Pseudomonas luteola, Escherichia coli, Klebseilla pneumoniae and Staphylococcus aureus. Susceptibility test were done by using VITEK 2 AST, P. luteola was showed sensitivity to most antibiotics but resistant to Cefazolin and Trimethoprim/ Sulfamethoxazole, but other gram negative bacteria were resistant to most antibiotics but sensitive to Gentamicin, Tobromycin, Ciprofloxacin. While gram positive bacteria (S. aureus) were detected as VERSA and MRSA, while sensitive to (Gentamicin, Tobramycin, Levofloxacin, Moxifloxacin, Linezolid, Nitrofurantoin, Tiglycine). While MIC determination of disinfectants such as, absolute alcohol, and bleach were made for the isolated bacteria by macro-dilution method. The highest MIC value against P. aeruginosa from alcohol was 64 mg/ ml. while the lowest MIC value of it was against K. pneumoniae, Staphylococcus aureus. On other hand the bleach gave the higher MIC value was against S. aureus, P. aeruginosa 25 mg/ml, 16 mg/ml respectively. But the lowest MIC value against E. coli. We were concluded that many pathogenic bacteria can be isolated from animal house and common between humans and animals and can cause diseases to workers and researchers in animal house. They must take the necessary precautions such as sterilization of various antiseptics to avoid infection with pathogenic bacteria.